Rasmussen J., 1984: A phosphorus 32 postlabeling assay for determining the incorporation of bromodeoxyuridine into cellular dna. Analytical Biochemistry: 525-528 Randerath’s procedure for 32p postlabeling of 3′-monophosphate deoxyribonucleotides from digests of cellular Dna has been modified. 3′-Monophosphate deoxyribonucleotides are converted to 3′,5′-bisphosphate deoxyribonucleotides with polynucleotide kinase and Atp; these products are enzymatically converted by P1 […]
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